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erα antibody (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology erα antibody
Erα Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1794 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/er%CE%B1+antibody/Estrogen+Receptor+alpha+Antibody/us12590950-294-30-32
Average 96 stars, based on 1794 article reviews

erα antibody - by Bioz Stars, 2026-09

96/100 stars

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Article Title: Muscle metabolic resilience and enhanced exercise adaptation by Esr1-induced remodeling of mitochondrial cristae-nucleoid architecture in males.
Article Snippet: Samples were transferred to PVDF membranes and subsequently probed with the following antibodies for protein and phospho-protein detection: ERα (Santa Cruz Biotechnology), Mfn1 (N111/24, Neuro Mab), GLUT4 and Mfn2 (Sigma-Aldrich), GAPDH (Millipore), pan actin, t-Akt/p-AKTSer473, IKK-β/p-IKKα/βSer180/181, JNK/p-JNKSer183/Tyr185, LC3B, tDRP1/pDRP1 Ser616/637 (Cell Signaling), FIS1 (Genetex), OPA1 (BD Biosciences), MitoProfile OXPHOS (MS604/ab110413; CI-NDUFB8 20kDa, CII-SDHB 30kDa, CIII-UQCR2 Core protein 2 48 kDa, CIV-MTCO140 kDa, and CV-ATP5A 55kDa) and Porin (Mitosciences, Abcam), PINK1 (Cayman Chemicals), p62 (Progen Biotechnik GmbH), and Parkin, GFP (Abcam).

Article Title: Pretreatment with estrogen enhances the therapeutic efficacy of cardiac progenitor cells and improves cardiac recovery in a failing heart model
Article Snippet: Following the transfer and blocking steps with 1% BSA in TBS-0.3% Tween, the membrane was incubated with the primer antibodies of either Col1a1 (Proteintech, 25870-1-AP), Nfatc3 (Thermo,PA5-78403), ERα (Santa Cruz,sc543) and ERβ (Santa Cruz,sc340243). β-Actin (Santa Cruz, sc-47778) primer antibody to use as house-keeping loading control.

Article Title: Muscle metabolic resilience and enhanced exercise adaptation by Esr1-induced remodeling of mitochondrial cristae-nucleoid architecture in males
Article Snippet: Samples were transferred to PVDF membranes and subsequently probed with the following antibodies for protein and phospho-protein detection: ERα (Santa Cruz Biotechnology), Mfn1 (N111/24, Neuro Mab), GLUT4 and Mfn2 (Sigma-Aldrich), GAPDH (Millipore), pan actin, t-Akt/ p -AKT Ser473 , IKK-β/p-IKKα/β Ser180/181 , JNK/p-JNK Ser183/Tyr185 , LC3B, tDRP1/p-DRP1 Ser616/637 (Cell Signaling), FIS1 (Genetex), OPA1 (BD Biosciences), MitoProfile OXPHOS (MS604/ab110413; CI-NDUFB8 20kDa, CII-SDHB 30kDa, CIII-UQCR2 Core protein 2 48 kDa, CIV-MTCO140 kDa, and CV-ATP5A 55kDa) and Porin (Mitosciences, Abcam), PINK1 (Cayman Chemicals), p62 (Progen Biotechnik GmbH), and Parkin, GFP (Abcam).

Incubation:

Article Title: Glucocorticoid induced loss of oestrogen receptor alpha gene methylation and restoration of sensitivity to fulvestrant in triple negative breast cancer
Article Snippet: .. Membranes were then blocked using 5 % skimmed milk for 1 hr, and then incubated with DNMT1, DNMT3a, β-actin antibodies (1:1000) (Cell Signalling), and ERα antibody 1:500 (Santa Cruz) in 5 % skimmed milk overnight on a shaker at 4 °C. .. Membranes were then washed with TBST (Sigma Aldrich) 3 times 10 min each, then incubated with appropriate secondary antibody (Ani-rabbit/ mouse 1:2000, Cell Signalling) and (Anti mouse/ goat 1:2000, Promega) in TBST for 1 h on a shaker at room temperature.

Article Title: Methods for predicting ER-mediated DNA damage
Article Snippet: .. Cells were washed with 1×PBS, blocked in 2% BSA/PBS with 0.1% Triton-X 100 for 1 hr at room temperature (RT), incubated overnight with γH2AX antibody (Cell Signaling #9718 S) or ERα antibody (Santa Cruz Biotechnology #sc-8002) at 4° C. and 1 hour with secondary antibody. .. S9.6: Cells were fixed in ice-cold 100% methanol for 10 min at −20° C., permeabilized in 100% acetone for 1 min at RT, blocked for 30 min in saline sodium citrate pH 7 (SSC, 4×), 3% BSA, 0.1% Triton-X and incubated with S9.6 (Kerafast #ENH001) 2 hr at RT followed by 1 h with secondary antibody.

Article Title: Transcription decouples estrogen-dependent changes in enhancer-promoter contact frequencies and spatial proximity
Article Snippet: .. Following 3x 2 min PBS rinses, slides were blocked with 1% BSA in PBS for 30 min at r.t. and incubated with 1:250 mouse anti ERα antibody (F-10, Santa Cruz, cat. Sc-8002) in 1% BSA in PBS for 1h. .. Slides were rinsed twice in PBS for 2 min and incubated for 45 min with 1:500 Goat anti-Mouse Alexa Fluor 488 antibody (Invitrogen, cat. A11001) in 1% BSA/ PBS.

Control:

Article Title: Female-specific dysfunction of sensory neocortical circuits in a mouse model of autism mediated by mGluR5 and estrogen receptor α
Article Snippet: .. Then, 10% of the lysate was set aside to be used as an input control and the rest was incubatedovernight at 4°C with 7 μL of ERα antibody or 1.4 μg of Homer antibody (Santa Cruz Biotechnology, D-3). .. The same isotype IgG was used as control for the specificity of the ERα (Abcam #ab172730) and Homer D3 antibodies.

Chromatin Immunoprecipitation:

Article Title: MicroRNA inhibitor therapy in systemic lupus erythematosus
Article Snippet: Cells were treated with 10 nM of 17β-estradiol (E2; Sigma-Aldrich, St. Louis, MO), 10 nM progesterone (Sigma), 10 ng/mL prolactin (Sigma), or 10 nM dihydrotestosterone (5α-androstatin-17β-ol-one; Fluka, Buchs, Switzerland) and were collected at the indicated times. (4) Chromatin Immunoprecipitation Sequencing (ChIP-Seq) MCF-7 cells were treated with 1 nM E2 for 45 min following 72 h culture in DMEM (Life Technologies) media containing 5% charcoal/dextran treated FBS. .. ChIP assays were performed using ERα antibody (Santa Cruz Biotechnology, Santa Cruz, CA). .. Sequencing libraries were prepared from ChIP enriched DNA using the Tru-seq Chip Sample Prep Kit (Illumina, San Diego, CA) according to the manufacturer's protocol.

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$Compression loading induces the expression of USP26 through phosphorylation of estrogen receptor-α at serine 118. Dual-luciferase reporter assays to detect Usp26 promoter activity ( a ) and quantitative analysis of Usp26 mRNA expression ( b ) were conducted in chondrocytes under compression stimulation at levels of 0 and 15 kPa for a duration of 4 h, with or without ER-α knockdown. c The common binding sites of ER-α on the Usp26 gene promoter were further analyzed and screened through three public databases: hTFtarget, Jaspar, and HOCOMOCO. d The binding site mutation significantly impaired the Usp26 promoter activity induced by compression loading in primary human chondrocytes. e ChIP‒qPCR analysis of the binding sites of ER-α to the Usp26 gene promoter in primary human chondrocytes. f Western blot to detect the phosphorylation of serine 118 of ER-α under compression loading in primary human chondrocytes. The phosphorylation-deficient S118A mutation significantly attenuated the compression-induced increases in Usp26 promoter activity ( g ) and mRNA expression ( h ) in primary human chondrocyte. i The S118A mutation significantly restored the decreased protein level of FBP2 caused by compression loading. Immunofluorescence staining to detect phosphorylation of ER-α at S118 in chondrocytes during skeletal growth ( j ), bone fracture healing ( k ), and osteoarthritis ( l ). White and Red scale bars indicated 500 μm. m Schematic of USP26 facilitates endochondral ossification by driving chondrocyte hypertrophy and mineralization. *** P < 0.001. P -values were analyzed by two-way ANOVA in a , b , d , e and one-way ANOVA in g , h$

Journal: Bone Research

Article Title: Ubiquitin-specific protease 26 facilitates endochondral ossification by driving chondrocyte hypertrophy and mineralization

doi: 10.1038/s41413-026-00517-5

Figure Lengend Snippet: Compression loading induces the expression of USP26 through phosphorylation of estrogen receptor-α at serine 118. Dual-luciferase reporter assays to detect Usp26 promoter activity ( a ) and quantitative analysis of Usp26 mRNA expression ( b ) were conducted in chondrocytes under compression stimulation at levels of 0 and 15 kPa for a duration of 4 h, with or without ER-α knockdown. c The common binding sites of ER-α on the Usp26 gene promoter were further analyzed and screened through three public databases: hTFtarget, Jaspar, and HOCOMOCO. d The binding site mutation significantly impaired the Usp26 promoter activity induced by compression loading in primary human chondrocytes. e ChIP‒qPCR analysis of the binding sites of ER-α to the Usp26 gene promoter in primary human chondrocytes. f Western blot to detect the phosphorylation of serine 118 of ER-α under compression loading in primary human chondrocytes. The phosphorylation-deficient S118A mutation significantly attenuated the compression-induced increases in Usp26 promoter activity ( g ) and mRNA expression ( h ) in primary human chondrocyte. i The S118A mutation significantly restored the decreased protein level of FBP2 caused by compression loading. Immunofluorescence staining to detect phosphorylation of ER-α at S118 in chondrocytes during skeletal growth ( j ), bone fracture healing ( k ), and osteoarthritis ( l ). White and Red scale bars indicated 500 μm. m Schematic of USP26 facilitates endochondral ossification by driving chondrocyte hypertrophy and mineralization. *** P < 0.001. P -values were analyzed by two-way ANOVA in a , b , d , e and one-way ANOVA in g , h

Article Snippet: With 10 μL of ER-α antibody (CST, #13258) or negative control anti-IgG (CST, #2729), fragmented chromatin (200 μg) was incubated overnight to form immune complexes, which were coupled to Protein A beads.

Techniques: Expressing, Phospho-proteomics, Luciferase, Activity Assay, Knockdown, Binding Assay, Mutagenesis, Western Blot, Immunofluorescence, Staining